Journal: PLOS ONE

Article Title: Anti-inflammatory effect of the combined treatment of LMT-28 and kaempferol in a collagen-induced arthritis mouse model

doi: 10.1371/journal.pone.0302119

Figure Lengend Snippet: (A) Arthritis scores of mice were measured every 3 days starting at 21 days after the first immunization. (B) DBA1/J mice showed deformation and swelling of the toe and ankle joints. (C) Paraffin-embedded sections of CIA mice joint were stained with hematoxylin and eosin. (D) Histological analysis was performed based on the analysis criteria: inflammation, synovial hyperplasia, pannus formation, and erosion of cartilage and bone. (E) The protein levels of IL-6, IL-1β and IL-17 in CIA mouse serum were measured using ELISA. Data are presented as mean ± SEM. # p <0.05 vs. normal group, * p <0.05, ** p <0.01, and *** p <0.005 vs. CIA control group.

Article Snippet: The cells were incubated with anti-IL-17A-PE antibody (Miltenyi Biotec) or anti-mouse FOXP3 antibody (Invitrogen, Waltham, MA, USA) diluted in 1× permeabilization buffer (eBioscience) for 30 min at 4°C in the dark.

Techniques: Staining, Enzyme-linked Immunosorbent Assay, Control

Journal: Annals of Translational Medicine

Article Title: Mesenchymal stromal cells ameliorate acute lung injury induced by LPS mainly through stanniocalcin-2 mediating macrophage polarization

doi: 10.21037/atm.2020.02.105

Figure Lengend Snippet: The secretion of IL-10 (left) and TSG6 (right) from STC2 silenced MSCs or control MSCs detected by ELISA. IL-10, interleukin 10; TSG6, tumor necrosis factor α stimulated gene 6. Data are shown as mean ± SD (n=3). *, P<0.05. STC2, stanniocalcin-2; MSC, mesenchymal stem cell; ELISA, enzyme-linked immunosorbent assay.

Article Snippet: Protein levels of interleukin-17A and TNF-α in the BALF were quantified by specific enzyme-linked immunosorbent assay (ELISA) kits (Boster Biological Technology Co., Ltd., China) according to standard protocols.

Techniques: Control, Enzyme-linked Immunosorbent Assay

Journal: Annals of Translational Medicine

Article Title: Mesenchymal stromal cells ameliorate acute lung injury induced by LPS mainly through stanniocalcin-2 mediating macrophage polarization

doi: 10.21037/atm.2020.02.105

Figure Lengend Snippet: STC2 contributed to MSCs-mediated ameliorating effects on LPS-induced ALI. Male adult BALB/c mice (8 to 10 weeks old) were randomly divided into four different groups: PBS + PBS, LPS + PBS, LPS + MSCshNTC, and LPS + MSCshSTC2. The ALI model was established by LPS (5 mg/kg) installation intranasally. The control group received the same volume of PBS. The mice were killed to obtain lung tissues 24 h after LPS treatment or were used to collect BALF. (A) The degree of lung injury was assessed with H&E staining; (B) histopathological mean lung injury scores; (C) neutrophil count in BALF; (D) the pulmonary permeability index was measured by Evans blue dye. TNF-α (E) and IL-17A (F) levels in BALF were detected using ELISA assay. n=10 each group. *, P<0.05; **, P<0.01; ***, P<0.001. STC2, stanniocalcin-2; MSC, mesenchymal stem cell; ALI, acute lung injury; BALF, bronchoalveolar lavage fluid; ELISA, enzyme-linked immunosorbent assay.

Article Snippet: Protein levels of interleukin-17A and TNF-α in the BALF were quantified by specific enzyme-linked immunosorbent assay (ELISA) kits (Boster Biological Technology Co., Ltd., China) according to standard protocols.

Techniques: Control, Staining, Permeability, Enzyme-linked Immunosorbent Assay

Journal: Annals of Dermatology

Article Title: Hyaluronan Oligosaccharides Improve Rosacea-Like Phenotype through Anti-Inflammatory and Epidermal Barrier-Improving Effects

doi: 10.5021/ad.2020.32.3.189

Figure Lengend Snippet: Treatment with hyaluronan oligosaccharides (oligo-HAs) reduced inflammatory cell infiltration, interleukin-17 (IL-17), and kallikrein 5 (KLK5) expressions. (A) Hematoxylin and eosin-stained tissue sections of LL-37 induced rosacea-like mice showed marked inflammatory cellular infiltration. Further treatments with oligo-HAs reduced cellular infiltration (H&E, ×100). (B) Majority of infiltrated cells were CD4+ T cells and infiltration of CD4+ T cells decreased in oligo-HAs treated group (CD4, ×100). (C) IL-17A expression was markedly increased in the epidermis of LL-37 induced rosacea-like mice. Oligo-HAs decreased the IL-17A expression regardless of the injection method (IL-17A, ×200). (D) Expression of KLK5 was increased in LL-37 induced rosacea-like mice (arrow head). Further treatment with oligo-HAs reduced KLK5 expression (KLK5, ×400). i.d.: intradermally, i.p.: intraperitoneally.

Article Snippet: To detect CD4, IL-17A, KLK5, filaggrin, and CD44 in histological specimens from mice, primary antibodies including a monoclonal mouse anti-CD4 antibody (BD Biosciences, Franklin Lakes, NJ, USA), monoclonal rat anti-mouse IL-17A antibody (Covalab, Villeurbanne, France), polyclonal rabbit anti-KLK5 antibody (Abcam, Cambridge, UK), polyclonal mouse anti-profilaggrin antibody (Covance, Princeton, NJ, USA), and monoclonal rat anti-human/mice CD44 antibody (eBioscience, San Diego, CA, USA) were used.

Techniques: Staining, Expressing, Injection